anti mouse igd biot  (SouthernBiotech)

Journal: iScience

Article Title: High affinity mAb infusion can enhance maximum affinity maturation during HIV Env immunization

doi: 10.1016/j.isci.2024.109495

Figure Lengend Snippet:

Article Snippet: Biotinylated Anti-mouse IgD , Southern Biotech , Cat #1120-08 RRID: AB_2631189.

Techniques: Blocking Assay, Recombinant, Staining, Transfection, Sequencing, Clinical Proteomics, Plasmid Preparation, Software, Magnetic Beads

Journal: Nature Communications

Article Title: The concerted change in the distribution of cell cycle phases and zone composition in germinal centers is regulated by IL-21

doi: 10.1038/s41467-021-27477-0

Figure Lengend Snippet: A – C WT and Il21r −/− mice were immunized i.p. with NP-KLH in alum and on day 7, spleen NP-binding GC B cells (CD138 − IgM − IgD − Gr1 − B220 + NP + IgG1 + CD38 − ) were sorted and RNA-seq performed. A Mean-difference plot of WT vs Il21r −/− GC B cells depicts genes that were significantly upregulated in WT compared to Il21r −/− GC B cells shown in red and significantly downregulated ones in blue (false discovery rate <0.05). B Gene expression fold changes in WT compared to Il21r −/− GC B cells. Significantly DE genes were ranked by FDR and the 50 lowest then ordered by FC, colored red if upregulated in WT compared to Il21r −/− , blue if downregulated. C , D limma barcode plots testing for GC zone signature or cMyc signature genes among DE genes from WT vs Il21r −/− GC B cells as in A . Genes were ranked left to right as most up in Il21r −/− to most up in WT GC. For zone signature ( C ), vertical red bars mark DZ signature genes while vertical blue bars mark LZ signature genes. Worms show relative enrichment of DZ and LZ signature genes and camera p values assess significance. The plot shows DZ genes enriched among those up in WT while LZ genes were enriched among those up in Il21r −/− GC. For the Myc signature ( D ), vertical red bars mark upregulated genes in Myc + cells while vertical blue bars mark genes upregulated in Myc − cells. Worms show relative enrichment of Myc signature genes and camera p values assess significance. The plot shows Myc +- associated genes were significantly up in WT GC B cells.

Article Snippet: GC B cell was enriched by magnetic sorting by staining with biotinylated antibodies to GR1 (clone 8C5, WEHI Antibody Facility; 1 in 200 dilution), IgD (clone 11-26, Southern Biotech, Cat. 1120-08; 1 in 200 dilution), CD4 (clone GK1.5, WEHI Antibody Facility; 1 in 200 dilution), CD8 (clone YTS.169, WEHI Antibody Facility; 1 in 200 dilution), and CD138 (clone 281-2, BD Biosciences, Cat. 553713; 1 in 200 dilution) followed by staining with anti-biotin microbeads (Miltenyi Biotec, Cat. 130-090-485; 1 in 200 dilution) and depletion using LS Columns (Miltenyi Biotec, Cat. 130-042-401) supported by a MACS separator magnetic stand (Miltenyi Biotec).

Techniques: Binding Assay, RNA Sequencing, Gene Expression